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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been observed in several tumors, which ends up in chromosome instability, and thus might lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a crucial part in promoting cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are explained. Two further splice variants have been reported but in-depth nucleotide sequence data isn't still offered.
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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized through the antibody.
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This guide outlines further more probable optimization of cellular and immunoassay parameters to be sure the very best outcomes are...
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This guideline outlines further possible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope about the target protein. By comparing the staining from the blocked antibody as opposed to the antibody by yourself, one can see which staining is certain.
Target Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterized by a dramatic periodicity in protein abundance throughout the mobile cycle. Cyclins function as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation patterns which contribute towards the temporal coordination of each and every mitotic celebration. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S changeover. This protein accumulates for the G1-S period boundary and is also degraded as cells development through S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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